Oil-free culture system for in vitro bovine embryo production
نویسندگان
چکیده
منابع مشابه
Improvement in Bovine Embryo Production In Vitro
Bovine oocytes were matured, fertilized, and cultured FCM 199 with serum and co-culture) in vitro (IVMFC) with addition, during different phases of the procedure, of antioxidants: superoxide dismutase (SOD) and reduced glutathione (GSH). The addition of SOD (1,500 or 3,000 IU/ml) did not improve proportions of oocytes undergoing cleavage or the development of embryos to morula and blastocyst st...
متن کاملBovine In vitro Embryo Production : An Overview
Dairy industry perfected the application of the first reproductive biotechnology, i.e. artificial insemination (AI) a great success story and also remains the user of embryo transfer technology (ETT). In addition, recently the researchers taking interest to embraced the field of Transvaginal Oocyte Recovery (TVOR) and in vitro production (IVEP) of embryos. IVF provides the starting point for th...
متن کاملHighly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System
Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...
متن کاملSerum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification.
The aim of this study was to examine the effects of co-culture with Vero cells during the in vitro maturation (IVM) and three culture media, B2+5% fetal calf serum (FCS) on Vero cells, synthetic oviduct fluid (SOF)+5% FCS, and SOF+20 gL(-1) bovine serum albumin (BSA), on the developmental competence of the embryos and their ability to survive vitrification/warming. We also tested the effect of ...
متن کاملReactive oxygen species in bovine embryo in vitro production.
Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Italian Journal of Animal Science
سال: 2010
ISSN: 1828-051X,1594-4077
DOI: 10.4081/ijas.2010.e32